I'm sure it's too late, but RoundUp-glyphosate works by killing amino acid creating bacteria in the soil, so you can't kill a plant growing in your tree's roots by spraying it without affecting the soil where your trees roots are, and therefore your tree.
Good old-fashioned salt is overlooked as a weed killer, salt water works great in the summer during dry times to kill the top of a tree, and you could put a small coke bottle half full of salt there and put the cut off stem of the Virginia creeper into it, it should suck the moisture out of the whole plant and dry out the roots.
But make sure you stand the bottle up, you could tape it to your tree cage.