Poaching and shooting from vehicles. They know. Two seasons ago I had my shooter buck come in on a scrape I'd made few minutes before. I chose not to shoot so I could instead watch him work this new scrape. At bottom of mountain,quarter mile awasy, at neighbors house, dude pulls in with his truck and they are joking and carrying on , shutting doors, doing usual. Buck up till now was completely relaxed, came to a stand still looking and listening in that direction for few minutes, became adjatated and soon took off as if those guys were beside him. He knew cars,people could be trouble. Funny thing tho I shot him couple wks later as I was taking piss from stand with my weiner hanging free. lol.
Also one year due to some knee trouble, I had to ride the ATV to stand each time thru season instead of walking. I repeatedly rode by deer that just looked at me as I passed. I stopped once on lee side of hill 40 yds from my stand, crossed ridge, and had to wait while a dozen deer fed acorns beneath the stand. I rode up top ridge couple wks ago after 4 mo of hunting season to do some hinge work. Rode to within just a few yards of 15 does, fawns, and bucks bedded at my hinge cut edge soaking up the sun, before they finally ran off.
My point, they know what to fear, especially the mature ones.